FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28502599
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA32850.015.555243550
CGCCGTA36050.015.04824147
GCCGTAT36250.014.77211748
GCGTCGT40650.013.86215412
CGCGAAA13350.012.58390643
GATCGGA47850.012.0695912
CCGTATC50350.011.12192249
GTCGCCG48500.010.96875645
CGTTCGC20650.010.50839316
CGACCGT12600.010.2782561
TAAGTCG25750.09.78614220
AGCGTCG58750.09.77039311
ATCGGAA60150.09.6014613
CCGGGAT53150.09.4171841
CGACCTA16750.09.4034564
CGTCGTG70150.09.13034613
TCGGGTC46450.09.1178032
TCGTGTA65250.09.065062515
CTCGTAT26000.09.019651
AGAGCGT64100.08.9549879
Produced by FastQC (version 0.11.5)