FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20807043
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA215300.062.28450412
CATACGA198650.062.2423915
GCATACG217400.061.48189514
ATACGAG196650.059.9879516
AGACGGC287250.057.4351169
AAGACGG290750.056.8777438
ACGGCAT245850.056.1810711
TACGAGA178900.055.22223317
CGAGATT181500.053.85439719
GACGGCA259800.053.53426710
ACGAGAT185000.053.3476618
CGGCGTG107350.052.3960712
ACGCGTG23250.052.26749846
GAAGACG315400.051.728987
GACGCGT24600.050.65838245
GGCATAC271050.049.71681213
ACGGCGT54200.048.5615311
GGCGCCG122900.045.7831244
AGCGGCG85950.044.98288710
GCGGCGT83500.043.48205611
Produced by FastQC (version 0.11.5)