FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30871023
Sequences flagged as poor quality0
Sequence length75
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG54350.025.96862643
TATGCCG60800.023.53176346
CGTATGC61050.023.43536244
CTCGTAT63000.023.29802142
ACACGTC71950.023.17704413
TATCTCG64350.022.0813439
ACCGATG77550.019.2912532
GATCGGA76450.018.6706711
TCTCGTA80500.018.42720641
CACACGT89500.018.28976812
CGATGTA84100.017.55812634
CACCGAT88350.016.96955931
CACGTCT95950.016.05332614
AGATCGG66800.016.0500661
ATGCCGT90000.015.93281847
CCGATGT93200.015.67102133
ATCTCGT92900.015.58941940
GCACACG113150.015.00877211
ACGTCTG103850.014.73904115
ATGTATC103400.014.08194436
Produced by FastQC (version 0.11.5)