FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30871023
Sequences flagged as poor quality0
Sequence length75
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA37000.028.13643650
GCGTCGT45000.023.56998412
CGCCGTA46000.022.63210347
GCCGTAT49900.020.79751648
GATCGGA55900.020.451552
TATCGCG9600.017.79560522
CCGTATC63950.016.58775549
AGAGCGT65050.016.5076229
AGATCGG65300.016.2498021
GTAGATC68500.016.20378932
AGCGTCG70750.015.22367511
CGTCGTG74600.014.92210813
TCGTGTA75850.014.67217115
GTATCAT72250.014.54509751
GTCGTGT75850.014.50302114
GGTCGCC78050.014.38940844
TCGCCGT74500.014.37083546
CGTGTAG77150.014.2978216
TTACGCG20000.013.95084554
TGGTCGC80550.013.57263643
Produced by FastQC (version 0.11.5)