FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29466093
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG68800.034.466943
TATGCCG70350.033.5690346
GATCGGA73050.032.703561
ACACGTC80250.032.0234513
CGTATGC74550.031.76578144
ATCGGAA85700.027.8746932
CTCGTAT87250.027.17848642
ACGTCTG94350.026.94698315
CACGTCT99350.025.762814
ATCTCGT98050.022.82570340
ATGCCGT103200.022.71814747
TCTCGTA99700.022.37940241
TCGGAAG109650.022.2836383
CACACGT111800.021.03374112
CGTCTGA124100.020.62291516
GCCGTCT113550.020.5587549
AATCTCG59550.020.31193239
GCACACG120600.020.21818211
CCGTCTT124850.018.97173750
GTATGCC127850.018.68446545
Produced by FastQC (version 0.11.5)