FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15243577
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA274260.17991840104196016TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA108900.036.3981971
TCGTATG103500.035.69898245
TATGCCG109350.034.25992248
ATCGGAA116100.034.137252
CGTATGC109550.033.75774846
ACACGTC117550.033.19595313
CTCGTAT106950.033.192644
ACGTCTG122650.031.64620215
AATCTCG112300.031.05863441
CACGTCT127150.030.71674714
TCTCGTA113350.030.46656843
TCGGAAG132300.029.9098743
ATCTCGT119400.028.98061642
ATGCCGT129500.028.7159349
CACACGT135950.028.55081412
CCGTCTT131100.028.44515852
GCACACG141950.027.68429611
GCCGTCT134600.027.67914251
CGTCTGA144650.026.976216
GTATGCC143000.026.51262347
Produced by FastQC (version 0.11.5)