FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32570516
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG74100.028.6408745
TATGCCG78000.027.07801448
CGTATGC83750.025.38136746
GATCGGA84650.024.389091
CTCGTAT88250.024.04859444
ACACGTC98800.023.23653213
AATCTCG95250.023.20962741
ACGTCTG107700.021.53739415
TCTCGTA99000.021.4716143
ATCGGAA97700.021.1261632
ATCTCGT106100.020.13094142
CACGTCT116300.020.09099814
ATGCCGT110800.018.7551849
GCCGTCT117950.017.90661451
CACACGT137400.016.90668312
CCGTCTT126650.016.70340352
TCGGAAG126300.016.6262073
GCACACG145100.016.45483411
TGCCGTC130750.016.23165150
CGTCTGA150300.015.523499516
Produced by FastQC (version 0.11.5)