FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39810873
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG45350.013.35299814
CGCTCGT57700.010.435986515
CGAAGAT120600.010.2168911
CGCAGAC92450.09.7472131
CGGACAT65600.09.6998171
TCGCGTA15350.09.52974755
CGGTAAT24450.09.4772641
CGACCGT16350.09.4482811
CCGGGAT79550.09.4321621
TAAGCGA26300.09.18383146
TTACACG44300.08.98476532
TCGTATG35950.08.89508945
CTCGGAT88850.08.8174591
CGCGACT12550.08.7922261
ACTCGTG69450.08.67006422
CGGAAAT70850.08.565751
CCCGATA73250.08.54538369
TTCGCGT18050.08.48118754
TAAGTCG34200.08.4550120
CGACGAT29200.08.4394321
Produced by FastQC (version 0.11.5)