FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30424485
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG62950.030.62855346
CTCGTAT63850.029.49447842
ACACGTC66700.029.07448813
GATCGGA65100.028.9827481
CGTATGC73300.026.30365644
TCGTATG78200.024.83193843
TCTCGTA77700.023.48246241
ATGCCGT81750.023.4583547
CACGTCT83600.023.23814814
ACTTAGG85500.022.91341432
ACGTCTG88650.022.10148215
CACTTAG92350.021.40053431
ATCTCGT88600.020.5547440
CACACGT96650.020.10065512
TTAGGCA100800.020.05159634
TCGGAAG97100.019.927463
CTTAGGC101200.019.7676533
ATCGGAA97600.019.330592
GCCGTCT107500.017.9997149
GCACACG116200.017.61306811
Produced by FastQC (version 0.11.5)