FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34823218
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA57950.043.1138650
GCGTCGT66500.039.19782312
GCCGTAT69850.036.12114348
CGCCGTA76700.033.1656747
GATCGGA81300.033.0935362
AGAGCGT89250.029.7882449
AGCGTCG93550.028.04104611
CCGTATC91000.028.02525349
GTAGATC94800.027.65633232
AGATCGG99000.026.7941441
TGGTCGC100450.025.5915243
GTATCAT104250.024.36335451
CGTGTAG111800.023.88735216
AAGAGCG113350.023.8519218
GTCGTGT111350.023.62790714
TCTCGGT111350.023.61111837
CGTCGTG113750.023.46205313
GAGTGTA106050.023.42531828
GGTCGCC110500.023.23294644
TCGCCGT110550.022.91239246
Produced by FastQC (version 0.11.5)