FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16425731
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG86700.042.19856343
TATGCCG89300.041.1982246
CGTATGC91100.040.3469544
GATCGGA99250.038.220611
CTCGTAT93500.037.8954342
ACACGTC100300.037.5970213
AATCTCG94850.036.7824439
ACGTCTG103600.036.46506515
TCTCGTA97500.035.57386441
ATCGGAA106900.035.4697232
ATGCCGT104800.034.94304747
CACGTCT110150.034.29669614
ATCTCGT102850.033.69040340
GCCGTCT109000.033.68910649
CCGTCTT112600.032.4914550
CACACGT118850.031.9859412
TCGGAAG120350.031.754373
TGCCGTC116800.031.38206548
GCACACG127800.030.17086611
CGTCTGA125100.030.00816516
Produced by FastQC (version 0.11.5)