FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16425731
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA25500.041.4464312
CATACGA20600.040.8788715
GCATACG27650.037.80668314
ATACGAG26700.028.36625116
AGACGGC54950.025.0011029
AAGACGG54500.024.8930348
ACGGCAT44900.023.38439611
GCCGTAT38750.022.68313447
CGTATCA38450.021.62779249
GATCGGA62650.021.6101651
GCGTCGT46550.021.45703511
GTCGCCG41900.020.81394644
CGCCGTA42300.020.77655646
ATCGGAA70750.019.2308752
AGCGTCG58850.019.05773210
GGCGCCG28550.019.01731544
GACGGCA60050.018.9039710
CCGTATC48400.018.30085848
GTAGATC62550.018.16181231
AGAGCGT62700.017.8862638
Produced by FastQC (version 0.11.5)