FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27607630
Sequences flagged as poor quality0
Sequence length75
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT21550.032.28889535
TACGTAA29350.024.49410837
TCGTATG48550.022.82750746
CCGTACG31650.022.50598134
GTACGTA32450.021.95113636
CGTAATC32250.021.77982939
ACCGTAC33200.021.65391233
TATGCCG54150.020.22344649
ACGTAAT35750.020.20098938
GATCGGA56700.019.950812
TATCGCG10300.019.8496122
CGTATGC57000.019.67493447
ACACGTC60650.019.5194114
AGATCGG62550.018.1426581
CTCGTAT66550.016.50392745
ACGTCTG73000.016.48795116
ATCGGAA70650.016.1046623
CACGTCT79600.015.12091515
TCTCGTA76550.015.08035644
CGTATCG22350.015.0510693
Produced by FastQC (version 0.11.5)