FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27607630
Sequences flagged as poor quality0
Sequence length75
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA34050.032.51795650
GCGTCGT36250.032.1980812
GCCGTAT41550.027.52991548
GATCGGA46700.026.0670812
AGAGCGT55700.021.9724489
TATCGCG8100.021.81614922
TGGTCGC48650.021.79831343
ATCGGAA59850.020.5067543
GTCGCCG53450.020.46381845
CGCCGTA57750.019.86498547
AGCGTCG60550.019.71680311
CCGTATC58500.019.2108449
AGATCGG62500.018.8362921
TCGCCGT56650.018.54329546
GTAGATC64450.018.00428432
CGTCGTG70450.017.6079613
GTCGTGT70800.016.95126714
TTACACG36300.016.90171432
TCTCGGT72950.016.36567737
AAGAGCG74700.016.3392168
Produced by FastQC (version 0.11.5)