FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24830709
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA64850.028.789661
ATCGGAA73300.025.553872
ACACGTC85550.025.07513613
ACGTCTG92550.023.362415
CACGTCT97500.022.38589314
TATCGCG5500.021.66820122
TCGGAAG94150.020.1483
CACACGT109050.019.7651812
GCACACG113900.019.49140511
CGTCTGA117300.018.22979416
GTCACGA40150.016.45253429
CGGAAGA137000.015.8818584
AGTCACG46200.015.32985428
AATCTCG53300.014.82138541
TCGTATG72600.014.63301243
CACGATC47400.013.86419731
TATGCCG76750.013.75521146
ACGATCA48850.013.731482532
TTACACG28100.013.69266132
CGTATGC78750.013.66492944
Produced by FastQC (version 0.11.5)