FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24830709
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA26750.025.53429450
GCGTCGT30350.023.4134612
TATCGCG5950.022.6105822
GCCGTAT31450.021.8275248
CGCCGTA34400.019.75504547
GATCGGA40050.019.4757862
CATACGA16700.019.20847715
CGGCATA18000.017.63081612
AGCGTCG41000.017.07930411
CCGTATC42150.016.85968649
ATCGGAA47950.016.6987553
AGAGCGT45750.016.2108339
GCATACG22000.015.67863214
GTCGCCG45900.015.10644745
TTACACG28350.015.09040832
TGGTCGC42150.015.05947143
CGAAGAT85650.014.1845561
GTCGTGT53300.013.59010814
CGTATCG14850.013.4798873
TCGCCGT46800.013.41531946
Produced by FastQC (version 0.11.5)