FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27558185
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA38450.030.68700250
GCCGTAT42800.027.7292348
CGCCGTA46500.025.745447
GCGTCGT50750.024.67617812
CCGTATC56350.021.4286949
AGCGTCG60950.021.05610811
TCGCCGT56450.019.61842746
TGGTCGC56300.019.24111243
GTCGCCG60700.019.2105345
GATCGGA73550.018.4360082
CGTGTAG73900.017.36368616
AGAGCGT74150.017.307919
TCGTGTA74150.017.25887715
GGTCGCC68450.017.13616244
ATCGGAA80650.017.1123753
CATACGA19400.016.89163215
CGTCGTG75300.016.81272713
GTAGATC75450.016.50660132
GTCGTGT78900.016.2200214
GTATCAT80950.015.81172951
Produced by FastQC (version 0.11.5)