FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29209695
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA40450.032.2398150
GCCGTAT47250.028.03795848
CGCCGTA49750.027.3224847
GCGTCGT53000.026.88378512
CCGTATC61850.022.14463449
TGGTCGC60650.020.87570243
AGAGCGT73000.020.2271949
GATCGGA75550.019.7292652
GTCGCCG69800.019.07821545
AGCGTCG78000.018.57670411
GTAGATC79700.018.48312432
GTCGTGT81400.018.30732714
CGTGTAG80850.018.26104516
TCGTGTA80550.018.20067615
TCGCCGT71850.018.05419246
GGTCGCC76000.017.70337544
TATCGCG6750.017.37662122
GTATCAT81200.017.03744951
ATCGGAA88800.016.9407923
CGTCGTG88800.016.78220713