FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32965155
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA37500.016.7626881
CGTATCG32450.014.3517163
GCGGTAT56650.013.2910741
CCCGTAT34300.013.191091
GCGTAAC51350.012.49560452
TCGTTCG53100.012.01882428
CGAGCGA83350.011.96129853
TTACACG34350.011.95114732
TTACGCG27000.011.75478754
GTATCGA29700.011.7313964
ACGGACA82750.011.63110518
ATTCCGT86050.011.42562853
GTTCGTT83050.011.00760830
GCGGTGT69650.010.9590941
TACGCGA30700.010.90003855
ATCGAAG67900.010.8217276
CATACGA17250.010.79914715
TCGGGTC79450.010.5510852
CGTTCGC21300.010.52733616
CGGACAG95200.010.4361519
Produced by FastQC (version 0.11.5)