FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26648092
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG38100.039.20683743
CGTATGC41400.036.08165744
TATGCCG42500.034.9848446
CTCGTAT52250.028.85293442
ACACGTC55600.028.42489213
GATCGGA53100.027.7643621
CACGTCT71200.022.48775514
ATGCCGT68150.022.07054147
ACGTCTG76050.020.68418715
CACACGT81050.019.79739412
ATCGGAA76000.019.2946172
ATCTCGT76050.018.55285640
TCGGAAG80650.018.3105033
GCACACG96250.017.60371611
GCCGTCT85700.017.55045149
CGTCTGA93900.017.04626716
TGCCGTC89550.016.9548
GTATGCC90200.016.82845545
GATCTCG86500.016.6305539
TATCGCG8000.015.09228422
Produced by FastQC (version 0.11.5)