FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30442472
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA23350.030.73442515
CGGCATA31950.028.29300712
GCATACG31600.027.62371614
ATACGAG37000.017.15792516
AGACGGC76100.014.0965819
ACGGCAT63050.013.78908911
AAGACGG76850.013.1057128
TCGCTCG32600.012.80608714
GCCGTAT39800.012.04583748
ATACGCG5056.0936145E-1011.61465216
CGTATCA41950.011.51409350
GACGGCA88000.011.36803710
TACGAGA49750.011.16558417
CGCCGTA44100.011.10542147
CGAAGAT90250.010.9697041
GTCGCCG43900.010.44834345
CGCGGGT22050.010.1686891
TAAGCGA20700.010.16321746
TCGGGTC45300.010.1273992
TTACACG32150.010.0936532
Produced by FastQC (version 0.11.5)