FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30734591
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG47900.041.77357546
TCGTATG51100.040.48003843
CGTATGC54850.037.7739144
ACACGTC68750.030.8292713
GATCGGA67800.030.2835561
CTCGTAT70650.029.75511642
ATGCCGT82550.024.9021947
CACGTCT89150.023.92582914
TCTCGTA84850.023.5446441
CACACGT95450.022.27607712
ATCTCGT96400.020.79350740
ACGTCTG106950.020.22257215
TCGGAAG100350.019.7644773
GCCGTCT104700.019.26145449
CGTCTGA119850.018.15865516
TGCCGTC114700.018.059248
TAATCTC111950.017.96544638
GCACACG124900.017.67123811
GTATGCC119000.017.63739845
GTAATCT112550.017.60025237
Produced by FastQC (version 0.11.5)