FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37602431
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG46950.021.60130343
CGTATGC52200.019.4943544
TATGCCG53700.019.39952546
CTCGTAT61450.016.84094442
TTACGCG38100.014.30497454
ACACGTC78850.013.21165813
GATCGGA75950.012.9633451
TACGCGA42850.012.7193155
GCGGTAT73150.012.0851591
TCTCGTA90200.011.39695741
TCGACCG55950.011.28355131
GTATCGA39850.011.2546154
CGACCGT55800.010.94293632
ATGCCGT97950.010.84687647
CGTATCG38250.010.8235433
ATCGGTA33500.010.81180718
CACGTCT99500.010.6777514
GTTACGC52950.010.618853
ATCGAAG82800.010.4577766
CCCGTAT37500.010.4465691
Produced by FastQC (version 0.11.5)