FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37602431
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAT71600.012.9134621
TTACGCG37350.012.28690854
GTATCGA37050.012.1965664
CGTATCG39800.011.4398243
TACGCGA41100.011.24982455
ATCGAAG80750.011.1932316
CCGACAT70450.010.8226151
TAAACGC66700.010.65686152
CGCGAAT17350.010.14125251
CCCGTAT40000.010.0912911
ATAGCCG42450.09.83223721
TCGACCG50600.09.81935931
ATCGGTA32400.09.7960918
CGCGTAA44750.09.7140071
CGACCGT50250.09.68117832
AATAACG52750.09.68115651
GTTACGC50150.09.4262253
CGCTTAT46000.09.37637329
GCGCTTA45050.09.34418528
CGCGGAT33350.09.2069351
Produced by FastQC (version 0.11.5)