FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36920645
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG65650.042.19400443
CGTATGC71350.038.7265244
TATGCCG72000.038.37700746
ACACGTC85200.033.44987513
CTCGTAT85600.032.03767442
GATCGGA90550.030.2725521
CACGTCT106100.026.76322714
TCTCGTA104500.025.81416341
ATGCCGT111450.024.79268347
ACGTCTG127700.022.47939915
ATCTCGT122700.022.04139540
CACACGT130000.022.02711712
TCGGAAG128400.021.7068543
ATCGGAA128800.021.2942242
GCCGTCT137600.019.80519749
GCACACG155850.019.2997111
AATCTCG141400.019.24842539
GTAATCT146400.018.3316237
TGCCGTC152250.018.26202248
GTATGCC162600.017.39650745
Produced by FastQC (version 0.11.5)