FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24955607
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG43750.034.69314643
CGTATGC44500.034.03090744
TATGCCG47200.032.23039246
CTCGTAT52500.028.91089442
GATCGGA54650.026.943571
ACACGTC61600.025.3689313
TCTCGTA66300.022.73715441
CACGTCT74950.020.85079214
ATGCCGT74400.020.44707547
ACGTCTG77800.020.04267715
ATCTCGT76900.019.6478940
ATCGGAA76350.019.1151542
CACACGT86000.018.25131412
TCGGAAG83850.017.93793
TATCGCG13850.017.43523222
GCCGTCT87350.017.29727249
GCACACG99950.016.46303211
CATCTCG94200.016.2226239
TGCCGTC96000.016.13401248
GTATGCC98550.015.54158845
Produced by FastQC (version 0.11.5)