FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28975739
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT15350.010.4915941
CCCGTAT30100.010.3517921
TACCGTA21800.010.2747959
CTCGGTT37350.09.7484321
CGAAGAT101400.09.5293471
CTCGGAT66350.09.3395171
TTACACG29250.08.97405632
CGCGACT9801.8189894E-128.9311221
CTCGGGT55050.08.9035411
CGTATCG21950.08.7698483
CGCGCTA16550.08.67095646
ACGGGCG49750.08.65301967
CGGGCGA47750.08.64896768
TCGCTCG37650.08.55249214
TAAGTCG21350.08.52549420
CTCGTTT44950.08.4117451
CGGGAAT55150.08.2525831
TCGCGCG5957.3277624E-68.2349458
ACCGTAT23450.08.20860710
TCGGATT37250.08.1745312
Produced by FastQC (version 0.11.5)