FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18642355
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG55450.032.5453951
TCGTATG61500.031.4707644
TATGCCG62900.031.04370747
CGTATGC63000.030.88582445
TATCTCG63900.030.07279640
GATCGGA61500.029.2301462
CTCGTAT67700.029.20001843
ACACGTC67150.028.97608814
AGTCACG66300.028.87895429
CGCCAAT74400.026.05945834
ACGTCTG77100.025.4603616
TCTCGTA77100.025.41622742
GTCACGC76050.025.17652330
ATGCCGT79400.024.76662448
ATCGGAA74200.024.3668443
CACGTCT79900.024.30906515
ATCTCGT83000.023.48495941
ACGCCAA85700.022.4623633
CACACGT89750.022.10219213
TCGGAAG87000.022.008584
Produced by FastQC (version 0.11.5)