FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32516535
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG39750.013.03201346
CGCGAAA13650.012.56466843
TCGTATG42000.012.41722543
CGCGCTA17850.011.96130446
CGTATGC43100.011.85679144
CTCGTAT48950.011.0832242
TCGGGTC53150.010.9960662
CTCGGTT36050.010.5024321
CGTTCGC19550.010.38258216
CTCGGGT71200.010.0443431
CGACCTA23000.010.0424624
CGCGTAT5653.369496E-89.9275681
CGCGTTC23300.09.16216314
ACACGTC56200.09.09160713
CCGGGAT54950.08.931651
CCCGGTT34550.08.8275991
TCGCGCG5957.7396E-78.8226378
TAAGTCG29200.08.74911520
CCGCGTT26100.08.71559313
CGCGAGT14650.08.6146221
Produced by FastQC (version 0.11.5)