FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49445045
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA82750.038.02271750
CGCCGTA91850.034.3031847
GCGTCGT93450.033.8448412
GCCGTAT93600.033.77139348
GATCGGA106900.030.5832372
CCGTATC118600.026.73934449
AGAGCGT126350.025.8518839
TGGTCGC126550.024.79037343
AGATCGG131550.024.5631791
CGTGTAG135950.024.12784216
GTAGATC133150.023.81310532
GTCGTGT138250.023.70312314
TCGTGTA138850.023.6738415
TCGCCGT133150.023.53369346
AGCGTCG138000.023.2188111
ATCGGAA140500.023.2182543
CGTCGTG139950.023.01974313
GGTCGCC139950.022.8094244
GTATCAT152200.021.0107151
AAGAGCG159700.020.6478888
Produced by FastQC (version 0.11.5)