FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23277064
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA21600.020.42633250
GCGTCGT26900.017.26985512
CGCCGTA30150.015.30853747
GCCGTAT30200.014.72194348
GATCGGA31700.014.2091642
GCGGTAT40000.014.0542731
CGTATCG24000.013.6878933
TTACGCG14900.013.43910154
CGCGTAT3559.5260475E-913.3554931
ATCGAAG47450.012.4193936
GTCGCTA19150.012.04345727
CATACGA11000.012.01271615
GTATCGA21300.011.9252654
TACGCGA17200.011.83785755
AGAGCGT42000.011.5452529
GCGGAAT27300.011.5366731
TATCGCG8050.011.37493822
CGCGTAA18850.011.1388551
CGGTCTA12950.011.003118544
CCCGTAT26350.010.9244251
Produced by FastQC (version 0.11.5)