FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30084814
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG33550.014.39593614
TCGTATG34400.014.04008145
TATGCCG38350.012.86384248
CGTATGC39700.012.51330446
CGCGTAT5003.8198777E-1112.4438591
CGCTCGT41750.011.40318715
CGTCCCG46350.011.38784935
CCGGGAT47450.011.3642541
CGCGAAT9800.011.2869461
CGAAGAT90600.011.0261071
TTACACG31400.010.32757132
CGTATCG21500.09.9511623
CTCGTAT49100.09.83663544
CGGGGAT54700.09.794831
TCGCGTA11050.09.67830855
ACCCGTC52450.09.66878432
CGCGGGT20100.09.6304041
ACTCGTG54150.09.3652422
GTATCGA30450.09.2920084
CGCAGAC69700.09.2738771
Produced by FastQC (version 0.11.5)