FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37259475
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG50800.012.47022614
CGTATCA37450.011.96231650
CGAAGAT130150.011.7246531
CGATAAC31500.011.3330631
CCGGGAT73900.010.987561
CGCGAAT17150.010.816071
CGCTCGT64500.010.20134815
CTCGGGT85100.010.1583951
GCCGTAT44300.09.95460348
CGTTCGC29000.09.89635616
CGCAGAC103400.09.8837111
CTCGGAT95750.09.83266451
TCGGGTC65350.09.80083752
CGCGAAC12650.09.6835641
ATATCGC24500.09.57117536
CGATCCA63900.09.4755174
TATATCG23300.09.46326835
GTCGTTA25550.09.31483420
CGCGTTC33200.09.06608614
CGCGTAT7001.0615622E-88.9997861
Produced by FastQC (version 0.11.5)