FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23386758
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT34850.018.90087711
GATCGGA51250.017.7381461
CGTATCA32000.017.3261849
GTCGCCG33850.016.89226244
GCCGTAT37450.015.723824547
CGCCGTA37950.015.15745746
ATCGGAA62950.014.332052
AGCGTCG48900.014.03709510
GTCGCTA14700.013.59470527
AGAGCGT53000.012.88260658
TCGCTCG26900.012.55590914
TGGTCGC41200.012.54245642
CCGGGAT33750.012.0394431
CCGTATC50150.011.74186948
AAGAGCG69950.011.6933157
GTAGATC62350.011.69001331
TCGCCGT45750.011.36880845
CGTCGTG59750.011.25628312
TCGGAAG79600.011.2478513
GGTCGCC55700.011.19339343
Produced by FastQC (version 0.11.5)