FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26967190
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA64500.031.3225141
ACACGTC84700.026.69433613
ATCGGAA78100.025.901822
ACGTCTG95850.023.90869115
CACGTCT102800.022.22604814
TCGGAAG100850.020.0250513
GCACACG120150.019.61162411
CACACGT116250.019.53742412
GTCACGA52150.019.06600829
AGTCACG57450.018.67031928
CGTCTGA124450.018.60573616
TCGTATG73950.018.14217243
TATGCCG75750.017.8038846
CGTATGC77600.017.4665944
TTACACG35100.016.78302432
CGGAAGA141850.016.2275374
ACGATCA61000.015.90912332
CACGATC61550.015.65631931
CTCGTAT87950.014.86711242
CGATCAG67200.014.74534633
Produced by FastQC (version 0.11.5)