FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33351431
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA38250.016.68508150
GCCGTAT40900.014.84287848
CGCCGTA42550.014.83337447
GCGTCGT47800.012.63172512
GTCGCCG51350.011.82035845
TCGCTCG41600.011.77734814
TTACACG36150.011.17326932
CCGTATC59800.010.90283749
TGGTCGC58400.010.86589143
CGTATCG21600.010.6991153
TCGCCGT57750.010.57011546
GTATCGA30700.010.5611144
CGAAGAT106950.010.4497691
CGCTCGT49800.010.11512915
AGCGTCG60300.09.89788111
TAAGTCG24800.09.734549520
CGCAGAC84300.09.6157411
CGTTCGC19650.09.48154516
GATCGGA66550.09.4331482
CCCGTAT34900.09.092951
Produced by FastQC (version 0.11.5)