FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28670398
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACACG32350.014.39714732
CGTATCG17100.012.9207193
GCCGTAT24050.011.76151948
CTCGGGT55600.011.6726211
CGCCGTA24250.011.66427347
CGAAGAT90000.011.6221231
GCGTCGT28300.011.21482812
TAAGTCG27500.011.16423120
TCGGGTC46800.010.990962
CGTATCA27050.010.96750850
TCGCTCG33850.010.90354714
CTCGGAT65400.010.8736361
CCCGTAT31800.010.8557111
GTATCGA24950.010.5160324
TCGGATT35350.010.4493572
CGCGTAT5402.1205778E-810.2284911
CGATAAG33050.010.22842317
CGGGGAT52150.010.1941671
CGACCTA20400.09.9846014
TACACGA46350.09.974339533
Produced by FastQC (version 0.11.5)