FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33690286
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG141800.036.943571
TCGTATG182100.030.42780146
GATCGGA178850.029.3992022
CGTATGC190150.029.0856447
TATGCCG192600.028.73386449
ACACGTC201600.028.47452714
CTCGTAT189100.028.18649145
ACGTCTG223350.025.97968116
TCTCGTA208800.025.56017744
CACGTCT224450.025.54495615
ATCGGAA213250.024.7538033
TCGGAAG226600.024.602934
ATCTCGT218300.024.57427443
ATGCCGT229550.024.09362650
CACACGT240600.024.01674813
AATCTCG208800.023.80862442
GCACACG266100.022.48045212
GCCGTCT243950.022.47334552
CGTCTGA268350.021.6872917
TGCCGTC261100.021.19542751
Produced by FastQC (version 0.11.5)