FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30731166
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG47200.013.199717546
CGTATGC48400.012.94476946
TATGCCG49100.012.76046949
CGTATCG23600.012.458183
TATCTCG59750.010.95437241
CCCGTAT33300.010.6186961
CCGGCAT51900.010.38837051
CCGGGAT70300.010.209221
CTCGTAT63500.010.1421544
CTCGGAT70850.010.1299671
GTATCGA32200.09.9993214
CGTTCGC20550.09.5372216
ACACGTC73600.09.41516813
GATCGGA64000.09.2973632
TCGGGTC51200.09.2973632
CGCGTTC23600.09.04605514
CGCGGGT25700.08.9909341
CCGTATC37050.08.8803592
CGCGAGT13950.08.783911
CTCGGGT65850.08.7724761
Produced by FastQC (version 0.11.5)