FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24237096
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT25100.019.65775348
CGTATCA25700.018.66140250
GCGTCGT27900.017.55636412
CGCCGTA31200.015.92496947
TATCGCG6650.014.0054820
GATCGGA42000.013.5593532
CCGTATC41050.012.69189249
TTACACG28750.012.23940732
GTCGCCG41200.012.14385845
CGACCTA16650.012.0231954
TGGTCGC38800.011.82694643
CTCGGAT61150.011.6266451
AGAGCGT44300.011.2905189
CGCGTAT4401.4523721E-710.9814341
AGCGTCG44800.010.93342711
ATCGGAA52250.010.8995423
CTCGGGT58200.010.6741471
GTATCGA22550.010.5610894
TATATCG12800.010.51064435
CGACTAA20050.010.49540328
Produced by FastQC (version 0.11.5)