FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29322467
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG21700.013.46312154
CGTATCG27100.013.1620663
GTATCGA28000.013.1029724
CCCGTAT29450.013.0687261
GCGGTAT60250.012.8324291
TACGCGA23450.012.45847555
TATCGCG8000.012.32575822
CGAGCGA53100.010.74781953
CGGTATT33400.010.3742762
ACGCGAC29350.010.30127956
ATCGAAG63400.010.2340656
CCGGTAT41100.010.1101621
GCGATCG42000.010.06082834
CGGGGTT76250.010.0503871
CGTATCA21400.010.000710550
ATCGGTA20050.09.99637318
CGCGTAA30350.09.9878361
CGCGGAT23750.09.7518141
CGTGCGA43850.09.63623431
CGCGTAT4654.122816E-69.5220511
Produced by FastQC (version 0.11.5)