FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53041143
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG101900.023.97042345
TATGCCG103150.023.58100348
CGTATGC109550.022.45180946
GATCGGA131100.018.8278661
CTCGTAT129000.018.72380344
AATCTCG134850.017.93676441
ACACGTC148650.017.21027613
ATCGGAA153850.016.0385422
TCTCGTA151450.015.9033843
ACGTCTG161000.015.86897715
ATCTCGT159350.015.15764542
CACGTCT176100.014.75940514
ATGCCGT168550.014.45139349
TCGCTCG70450.013.81083414
GCCGTCT186750.013.24339251
TCGGAAG197350.012.9685633
GCACACG211500.012.57856811
CCGTCTT196050.012.56310952
CGTCTGA210550.012.47366416
CACACGT208050.012.44376512
Produced by FastQC (version 0.11.5)