FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32398855
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG39050.014.22565614
CATACGA16350.012.02882515
CGCTCGT49050.011.39572915
CGCGAAC8400.010.67657851
CGTATCG18700.09.7758583
GCATACG21550.09.76674814
CGCAGAC83300.09.7310761
TCGTGCG58050.09.68381124
ACTCGTG60650.09.66658422
CGAAGAT94300.09.5469991
CTCGGGT67900.09.49971
CGTTCGC19050.09.41834316
CGGCATA21300.09.23342212
TCGGGTC52350.09.1585092
CTCGGTT40800.09.1306491
TACTCGT62150.09.10038221
CTCGTAC64100.09.043117
CGCGGAT20350.08.9835821
TAAGCGA22000.08.93517546
GCTCGTA64800.08.8389216
Produced by FastQC (version 0.11.5)