FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29142709
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG35550.013.00341943
TCGCTCG39700.012.94752214
TATCGCG7250.012.37203520
TATGCCG44200.010.77084946
CCGGGAT54850.010.5922661
CTCGTAT46900.010.59209942
CGAAGAT92250.010.5715581
CGTATGC45100.010.32646944
TTACACG31100.010.31610332
CGCGAAT11700.09.7540351
ACTCGTG57450.09.60883622
CGTTCGC20250.09.54015416
CGCGTAA5603.828136E-79.2631671
CGCAGAC85300.09.243621
CTCGGAT67450.09.1775621
ACCCGAT55000.09.15783968
CCCGATA54700.09.14469769
CGCGGAT25750.09.1324631
ACACGTC55650.09.112613
CGCTCGT59400.09.06006415
Produced by FastQC (version 0.11.5)