FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29142709
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG6800.012.68351920
TCGCTCG44200.011.55177814
TTACACG31100.011.53664132
CGAAGAT91650.011.3475441
CCGGGAT53650.011.334721
CTCGGAT68200.010.3857361
CGCCAAT54400.09.9716861
CGCGTAT6251.9908839E-89.3980281
CGGGAAT53650.09.2094591
CGGAGAT75250.09.0913111
CTCAGAT173350.09.0689161
CGCGAAT12000.08.9258231
CGCGGAT24500.08.8846911
CTCGGGT67600.08.7912291
CGCGATT9052.3646862E-118.7810631
CGCGCAT12600.08.77500251
CGCAGAC89200.08.7540991
CCGGAAT53350.08.6783711
CGCAAAT35100.08.6625031
TAAGTCG24700.08.65971120
Produced by FastQC (version 0.11.5)