FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37612446
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG66350.017.19668414
CGCTCGT75850.015.13513515
CGCAGAC107000.014.163551
CGTATCA57750.013.09090950
GCCGTAT61600.012.38636348
GATCGGA96800.012.3295451
CCGCGTT29850.012.07705213
ACTCGTG96250.011.92727322
CGCCTCG99850.011.707561529
CGCGTTC29500.011.62711814
TCGTGCG99800.011.60821624
CTCGTAC99000.011.5959617
GCTCGTA103800.011.53179216
CATACGA24000.011.52083315
CGTGCGC104600.011.30975125
ACTTCGC105200.011.14543711
AACCCGA109300.011.04757667
GCATACG29550.011.01522914
CCGGGAT65550.010.9458431
CCCGATA104400.010.92911969
Produced by FastQC (version 0.11.5)