FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30174260
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA38900.020.752349
GCCGTAT42350.019.30611847
CGCCGTA47050.017.45087846
CCGTATC54500.014.81219248
GCGTCGT60000.014.43186411
GATCGGA71800.013.1885861
TCGCTCG40350.012.99648514
AGCGTCG75050.012.87085210
TCGCCGT63550.012.10568245
AGAGCGT82250.011.7861078
ATCGGAA81600.011.6288692
GTAGATC75700.011.62113731
TGGTCGC63750.011.52655142
GTCGCCG73000.010.86938444
CGTCGTG81900.010.78353312
CCGGGAT68350.010.7193761
GGTCGCC80000.010.60828343
GTCGTGT79700.010.38912113
TCGTGTA81000.010.35016214
CGACAAT32800.010.3258151
Produced by FastQC (version 0.11.5)