FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22080657
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG29000.012.82284214
CGAAGAT72250.012.0313941
CCGGGAT43000.011.6992061
CGCAAAT27750.011.0637171
CGCGAAT10000.010.727141
TCGTATG22250.010.21317143
TATGCCG26700.010.18738146
CGCAGAC62200.010.1098451
TTACACG25300.010.07052232
TATCGCG4452.046987E-610.0587920
CGGACAT39700.09.876461
CGCGTAT3408.4520795E-49.7915071
CGGGATT22500.09.6996361
ATTAGCG9750.09.5345236
AATCGGG31050.09.4253425
TAAGTCG18050.09.34719620
CGGGGAT45200.09.2475351
CTCGGAT56450.09.1738171
TCGATTG22600.09.14124320
CGTATGC28100.09.06717744
Produced by FastQC (version 0.11.5)