FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22080657
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG32800.015.14732614
CGAAGAT73050.011.81660651
CGCAGAC68800.011.5930191
TTACACG26050.011.25696132
TAAGCGA14500.010.94459846
CGCTCGT45700.010.7206115
CTCGTAC47350.010.71136217
ACTCGTG46600.010.5867622
CGGGGAT44750.010.5706221
CGACTAA19800.010.28013428
CCCGATA45400.010.25836369
TATATCG11550.010.1556335
CGCCAAT40100.09.8159751
CCGGGAT42850.09.7500661
ACTTCGC54550.09.67624111
TCGTGCG51750.09.59984124
CGACCGT13400.09.5338861
GTATCGA21200.09.4389934
ATTAGCG9550.09.3924726
GCTCGTA54650.09.34369916
Produced by FastQC (version 0.11.5)