FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29160997
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA37200.021.4513649
GATCGGA64650.020.2478051
GCCGTAT40050.020.187247
ATCGGAA72900.017.8123512
CGCCGTA45950.017.21442846
GCGTCGT49050.016.12651611
AGCGTCG62150.015.99367710
CCGTATC54550.014.82124348
AGAGCGT69350.014.4845768
GTCGCCG48050.014.13121344
CATACGA15400.014.09088915
GTAGATC68650.014.0201831
AAGAGCG89850.013.5174637
TCGGAAG100300.012.7020943
CGGCATA20850.011.24706312
ATCTCGG104950.011.20537835
GTATCAT73250.011.08530850
TTACACG35950.011.00124332
CGACCTA22200.010.7208894
CGCGAAA13400.010.7090543
Produced by FastQC (version 0.11.5)